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XuJinzhu,QinChangsheng,YangHua,ZhaoDanyang,JieYuze,QiuHualong
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Production and dynamic of destruxins from Metarhizium spp.
Xu Jinzhu,Qin Changsheng,Yang Hua,Zhao Danyang,Jie Yuze,Qiu Hualong
(Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization/Guangdong Academy of Forestry, Guangzhou,)
Abstract:
Taking the yield of DxtA and DxtB as index- es, the difference of the destruxin producing ability of 15 Metarhizium spp. strains (belonged to 5 species) was analyzed. The DxtA dynamic curve of the best strain was fitted under shaking flask culture by sampling every day. The results showed that there was a significant dif- ference in the destruxin producing ability among 5 gen- era. Metarhizium anisopliae strains showed the highest production, M. lepidiotae produced a little Dxts, while M. flavoviride, M. taii and M. acridum did not produce DxtA or DxtB. Among them, the M19 strain was the best to produce DxtA (144.12 μg/mL), and the M25 strain was the best to produce DxtB (65.31 μg/mL). The M25 was the best in DxtA and DxtB production. Under shaking flask culture, the DxtA production of M25 was fitted by SPSS, and showed to fit a Logist model(Y =84.99/(1+11.82×e -0.3X ). In the first four days, the M25 was in vegetative growth period and produced a little Dxts. From the fifth day, it changed into exponential growth and reached a plateau from the ninth day.
Key words:  Meterhizium · Destruxin · Dynamics curve

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