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SuLingye,ZhangBowen,WangHongfeng,HeChunmei,XuQiaolin,ZengLei
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Root morphology and biomass accumulation in hairy root culture ofPsammosilene tunicoides by exogenous auxin treatments
Su Lingye,Zhang Bowen,Wang Hongfeng,He Chunmei,Xu Qiaolin,Zeng Lei
(Guangdong Provincial Key Laboratory of Silviculture Protection and Utilization/ Guangdong Academy of Forestry, Guangzhou,;First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou,)
Abstract:
Root morphology, growth rate and biomass accumulation in hairy root culture could be impacted by auxins. In this study, we investigated the responses of the morphology and growth of Psammosilene tunicoides hairy roots to different treatments of auxins at several concentrations in solid or liquid media. Results show that Naphthylacetic acid (NAA) principally accelerates the P. tunicoides primary or lateral hairy root elongation, while indolebutyric acid (IBA) mainly increases later- al hairy root density. The biomass of hairy roots was generally promoted by NAA, IBA or NAA/IBA com- bination. The optimal concentrations of NAA, IBA and NAA/IBA for root biomass enhancements are 0.5, 0.1, 0.5/0.2 mg/L for solid media, and 0.1, 0.1, 0.05/0.2 mg/ L for liquid media, respectively. This study positively suggests that the root morphology of P. tunicoides hairy root can be improved by optimizing the ratio of NAA/ IBA in the culture media, leading to an improving bio- mass in hairy root culture.
Key words:  Hairy root · IBA · NAA · Optimum concen- tration · Psammosilene tunicoides

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