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猴耳环嫩枝和叶组织的代谢物分析
黄婷1, 彭冠明1, 林昌明1, 司徒荣贵1, 毛积鹏2
1.台山市红岭种子园;2.台山市红岭种子园/华南农业大学
摘要:
对猴耳环 Pithecellobium clypearia 主要药用部分嫩枝和叶中的代谢物成分及其含量差异进行分析,从猴耳环的嫩枝和叶中共鉴定出 712 种代谢物成分,主要为氨基酸类、有机酸类、酚酸类、脂质类和黄酮类等化合物。猴耳环嫩枝和叶组织部位之间代谢物成分含量差异显著,其中槲皮素、表儿茶素苷和表没食子儿茶素没食子酸酯等猴耳环药用特征标志物在嫩枝中的含量显著高于叶组织,而表儿茶素、槲皮苷和杨梅苷等特征标志物则在叶组织中含量更高。综合表明猴耳环嫩枝和叶组织均富含多种高生物活性物质,大部分药用特征代谢物在嫩枝和叶中的含量差异显著。
关键词:  猴耳环  UPLC-MS/MS  代谢物  嫩枝  叶片
DOI:
分类号:
基金项目:中央财政林业科技推广示范项目(〔2018〕GDTK -14 号,2018-2021)
Metabolites Analysis of the Twigs and Leaves of Pithecellobium clypearia
Huang ting1, Peng guan ming1, Lin chang ming1, Si tu rong gui1, Mao ji peng2
1.Taishan Hongling Seed Orchart;2.Taishan Hongling Seed Orchart/South China Agricultural University
Abstract:
The metabolites compositions and content differences in the twigs and leaves of Pithecellobium clypearia were analyzed. The results showed that total of 712 metabolites were identifified, including the amino acids, organic acids, phenolic acid, lipids and flflavonoids. In addition, the results showed that there were signifificant differences in the content of metabolites between twigs and leaves. For example, the contents of quercetin, epicatechin glucoside and epigallocatechin gallate in twigs were significantly higher than in leaves, while the contents of epigallocatechin, quercitrin and myricetrin were higher in leaves. These results showed that both twigs and leaves of P. clypearia were rich in various high bioactive substances, and there were signifificant differences in the contents of most medicinal characteristic metabolites in twigs and leaves.
Key words:  Pithecellobium clypearia  UPLC-MS/MS  metabolites  twigs  leaves