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药用植物猴耳环的转录组测序分析
谭志强, 黄炬峰, 李福明, 邓乐平, 毛积鹏
台山市红岭种子园
摘要:
利用 Illumina HiSeq 4 000 对猴耳环 Pithecellobium clypearia 的主要药用部位嫩枝和叶进行 了转录组测序分析。共获得 24 748 936 个高质量的 reads,拼接组装到 63 299 个 Unigenes,平均长度为 1 117 bp。其中 26 101 个 Unigenes 在 Nr、UniProt、KOG、GO 和 KEGG 数据库获得注释信息。鉴定到了 192 个和黄酮类化合物生物合成相关的 Unigenes,主要涉及了苯丙烷、黄酮、异黄酮、黄酮醇和花青素等 化合物的生物合成通路。此外还鉴定到了 136 个和萜类化合物生物合成相关的 Unigenes,主要参与了萜类 骨架、单萜、倍半萜、二萜和四萜等化合物的生物合成通路。最后在 15 740 个 Unigenes 中检测到 45 573 个 SSR 标记,出现频率为 33.84%。综合表明猴耳环嫩枝和叶的转录组测序结果可靠,SSR 标记丰度高。
关键词:  猴耳环  转录组测序  萜类化合物  黄酮类化合物  SSR 标记
DOI:
分类号:
基金项目:
Transcriptome Sequencing and Analysis of the Medicinal Plant Pithecellobium clypearia
Tan zhi qiang, Huang ju feng, Li fu ming, Deng le ping, Mao ji peng
Taishan Hongling Seed Orchart
Abstract:
Transcriptome sequencing was performed on the twigs and leaves of Pithecellobium clypearia based on Illumina HiSeq 4 000 platform. Total of 24 748 936 high quality reads were obtained by RNA-Seq to the mixed samples of twigs and leaf tissues of P. clypearia, and assembled into 63 299 unigenes with an average length of 1 117 bp. 26 101 unigenes were annotated in five databases. 192 unigenes related to the flavonoids were identified, mainly involved in the biosynthesis pathways of flavones, isoflavones, flavonols, anthocyanins and phenylpropane. Moreover, 136 unigenes related to the terpenoids were identified, mainly participated in the biosynthesis pathways of terpenoid backbone, monoterpenes, sesquiterpenes, diterpenes and triterpenes. Finally, 45 573 SSR markers were detected in 15 740 unigenes, with a frequency of 33.84%. These results showed that the RNA-Seq results of twigs and leaves of P. clypearia were reliable, and rich in SSR markers.
Key words:  Pithecellobium clypearia  transcriptome  terpenoids  flavonoids  SSR markers